BD-PT2772-MKP-1/2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MKP-1/2 Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT2772

产品名称

MKP-1/2 Rabbit Polyclonal Antibody

别名

DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4;

类别

常规抗体

基因名称

DUSP1/4

蛋白名称

Dual specificity protein phosphatase 1/4

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1843/1846

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1843

Human Swissprot No.

P28562/Q13115

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P28562/entry

Mouse Gene ID

19252/319520

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19252

Rat Gene ID

114856/60587

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=114856

Rat Swissprot No.

Q64623/Q62767

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q64623

免疫原

The antiserum was produced against synthesized peptide derived from human MKP-1/2. AA range:261-310

特异性

MKP-1/2 Polyclonal Antibody detects endogenous levels of MKP-1/2 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

41kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferati

组织表达

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

细胞定位

Nucleus .

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;

功能

catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.,induction:By oxidative stress and heat shock.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.,similarity:Contains 1 rhodanese domain.,similarity:Contains 1 tyrosine-protein phosphatase domain.,tissue specificity:Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MKP-1/2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using MKP-1/2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using MKP-1/2 antibody.

Western Blot analysis of various cells using MKP-1/2 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of Jurkat cells using MKP-1/2 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of 293T lysis using MKP-1/2 antibody. Antibody was diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of Hela KB 293T SH-SY5Y lysis using MKP-1/2 antibody. Antibody was diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.