产品名称
MGMT Rabbit Polyclonal Antibody
别名
MGMT; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
蛋白名称
Methylated-DNA--protein-cysteine methyltransferase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4255
Human Swissprot No.
P16455
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P16455/entry
Mouse Swissprot No.
P26187
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P26187
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25332
Rat Swissprot Link
http://www.uniprot.org/uniprot/P24528
免疫原
The antiserum was produced against synthesized peptide derived from human MGMT. AA range:1-50
特异性
MGMT Polyclonal Antibody detects endogenous levels of MGMT protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015],
组织表达
Cerebellum,Epithelium,Placenta,
功能
catalytic activity:DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.,cofactor:Binds 1 zinc ion.,function:Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.,similarity:Belongs to the MGMT family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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