产品名称
MEK Kinase-3 Rabbit Polyclonal Antibody
别名
MAP3K3; MAPKKK3; MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3
蛋白名称
Mitogen-activated protein kinase kinase kinase 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4215
Human Swissprot No.
Q99759
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99759/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26406
Mouse Swissprot No.
Q61084
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61084
免疫原
The antiserum was produced against synthesized peptide derived from human MAP3K3. AA range:101-150
特异性
MEK Kinase-3 Polyclonal Antibody detects endogenous levels of MEK Kinase-3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
组织表达
Aorta,Brain,Epithelium,Kidney,Melanoma,Placenta,PNS
信号通路
Regulation of Actin Dynamics; SAPK_JNK; Cell Growth; Stem cell pathway; B Cell Receptor
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-530.,function:Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
