产品名称
MDFI Rabbit Polyclonal Antibody
别名
MDFI; MyoD family inhibitor; Myogenic repressor I-mf
蛋白名称
MyoD family inhibitor
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4188
Human Swissprot No.
Q99750
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99750/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17240
Mouse Swissprot No.
P70331
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P70331
免疫原
The antiserum was produced against synthesized peptide derived from human MDFI. AA range:103-152
特异性
MDFI Polyclonal Antibody detects endogenous levels of MDFI protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008],
细胞定位
Nucleus . Cytoplasm .
功能
function:Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.,similarity:Belongs to the MDFI family.,subunit:The C-terminus interacts with AXIN1 and LEF1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
