BD-PT2679

Mcl-1 Rabbit Polyclonal Antibody

MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

常规抗体

MCL1

Induced myeloid leukemia cell differentiation protein Mcl-1

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

4170

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4170

Q07820

http://www.uniprot.org/uniprotkb/Q07820/entry

17210

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17210

P97287

http://www.uniprot.org/uniprot/P97287

60430

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=60430

Q9Z1P3

http://www.uniprot.org/uniprot/Q9Z1P3

The antiserum was produced against synthesized peptide derived from human MCL1. AA range:91-140

Mcl-1 Polyclonal Antibody detects endogenous levels of Mcl-1 protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

About 40kd in human,39kd in mouse and rat

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],

Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th

Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.

function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.,similarity:Belongs to the Bcl-2 family.,subcellular location:Cytoplasmic, associated with mitochondria.,subunit:Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptototic BCL2-related proteins.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.