产品名称
MBD3 Rabbit Polyclonal Antibody
别名
MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
蛋白名称
Methyl-CpG-binding domain protein 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=53615
Human Swissprot No.
O95983
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O95983/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17192
Mouse Swissprot No.
Q9Z2D8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z2D8
免疫原
The antiserum was produced against synthesized peptide derived from human MBD3. AA range:221-270
特异性
MBD3 Polyclonal Antibody detects endogenous levels of MBD3 protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013],
组织表达
Brain,Cervix,Eye,Muscle,Uterus,
细胞定位
Nucleus. Chromosome. Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.
功能
function:Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.,similarity:Contains 1 MBD (methyl-CpG-binding) domain.,subcellular location:Nuclear, in discrete foci.,subunit:Heterodimer with MBD2. Part of the NuRD and the MeCP1 complex. Binds HDAC1, MTA2, DNMT1, p66-alpha and p66-beta.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
