BD-PT2663-MMP-7 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MMP-7 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2663

产品名称

MMP-7 Rabbit Polyclonal Antibody

别名

MMP7; MPSL1; PUMP1; Matrilysin; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase

类别

常规抗体

基因名称

MMP7

蛋白名称

Matrilysin

推荐应用

反应种属

Human,Mouse,Rat,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4316

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4316

Human Swissprot No.

P09237

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P09237/entry

Mouse Swissprot No.

Q10738

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q10738

Rat Gene ID

25335

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25335

Rat Swissprot No.

P50280

Rat Swissprot Link

http://www.uniprot.org/uniprot/P50280

免疫原

The antiserum was produced against synthesized peptide derived from human MMP-7. AA range:218-267

特异性

MMP-7 Polyclonal Antibody detects endogenous levels of MMP-7 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

29kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

matrix metallopeptidase 7(MMP7) Homo sapiens This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016],

组织表达

Colon,Human small intestine,Kidney,Placenta,

细胞定位

Secreted, extracellular space, extracellular matrix .

信号通路

WNT;WNT-T CELL

功能

catalytic activity:Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).,cofactor:Binds 2 calcium ions per subunit.,cofactor:Binds 2 zinc ions per subunit.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,function:Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.,similarity:Belongs to the peptidase M10A family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MMP-7 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, using MMP-7 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using MMP-7 antibody.

Western Blot analysis of various cells using MMP-7 Polyclonal Antibody diluted at 1：500

Western Blot analysis of COS7 cells using MMP-7 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human uterus. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.