产品名称
MARK2 Rabbit Polyclonal Antibody
别名
MARK2; EMK1; Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b; Par-1b; Par1b
蛋白名称
Serine/threonine-protein kinase MARK2
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2011
Human Swissprot No.
Q7KZI7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q7KZI7/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13728
Mouse Swissprot No.
Q05512
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q05512
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=60328
Rat Swissprot Link
http://www.uniprot.org/uniprot/O08679
免疫原
The antiserum was produced against synthesized peptide derived from human MARK2. AA range:10-59
特异性
MARK2 Polyclonal Antibody detects endogenous levels of MARK2 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
microtubule affinity regulating kinase 2(MARK2) Homo sapiens This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009],
组织表达
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
细胞定位
Cell membrane; Peripheral membrane protein. Cytoplasm. Lateral cell membrane. Cytoplasm, cytoskeleton. Cell projection, dendrite . Cytoplasm . Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm.
信号通路
Regulation of Microtubule Dynamics
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.,tissue specificity:High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
