产品名称
MARCH5 Rabbit Polyclonal Antibody
别名
MARCH5; RNF153; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153
蛋白名称
E3 ubiquitin-protein ligase MARCH5
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54708
Human Swissprot No.
Q9NX47
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9NX47/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=69104
Mouse Swissprot No.
Q3KNM2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q3KNM2
免疫原
The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70
特异性
MARCH5 Polyclonal Antibody detects endogenous levels of 40607 protein.
稀释度
WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008],
组织表达
Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.
细胞定位
Mitochondrion outer membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301). .
功能
domain:The RING-CH-type zinc finger domain is required for E3 ligase activity.,function:Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology. Promotes ubiquitination of DRP1. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-CH-type zinc finger.,subunit:Interacts with MTFN2 and ubiquitinated forms of DRP1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
