产品名称
MAPKAPK-2 Rabbit Polyclonal Antibody
别名
MAPKAPK2; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2
蛋白名称
MAP kinase-activated protein kinase 2
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9261
Human Swissprot No.
P49137
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49137/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17164
Mouse Swissprot No.
P49138
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P49138
免疫原
The antiserum was produced against synthesized peptide derived from human MAPKAPK2. AA range:238-287
特异性
MAPKAPK-2 Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Expressed in all tissues examined.
细胞定位
Cytoplasm . Nucleus . Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;VEGF;Neurotrophin;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Seems to be activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors, the second, triggered by stress and heat shock, depends on the activation of MPK2 and upstream MAPKK/MAPKKK.,function:Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.,PTM:Phosphorylated and activated by MAP kinase.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PHC2.,tissue specificity:Expressed in all tissues examined.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
