产品名称
Cleaved-Factor Xa activated HC (I235) Rabbit Polyclonal Antibody
别名
F10; Coagulation factor X; Stuart factor; Stuart-Prower factor
蛋白名称
Coagulation factor X
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2159
Human Swissprot No.
P00742
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P00742/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14058
Mouse Swissprot No.
O88947
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O88947
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29243
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63207
免疫原
The antiserum was produced against synthesized peptide derived from human FA10. AA range:216-265
特异性
Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody detects endogenous levels of fragment of activated Factor Xa activated HC protein resulting from cleavage adjacent to I235.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative sp
组织表达
Plasma; synthesized in the liver.
信号通路
Complement and coagulation cascades;
功能
catalytic activity:Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.,function:Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.,online information:Factor X entry,PTM:N- and O-glycosylated.,PTM:The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).,PTM:The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.,PTM:The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 Gla (gamma-carboxy-glutamate) domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 2 EGF-like domains.,subunit:The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds.,tissue specificity:Plasma; synthesized in the liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
