产品名称
mAChR M2 Rabbit Polyclonal Antibody
别名
CHRM2; Muscarinic acetylcholine receptor M2
蛋白名称
Muscarinic acetylcholine receptor M2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1129
Human Swissprot No.
P08172
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P08172/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=243764
Mouse Swissprot No.
Q9ERZ4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9ERZ4
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81645
Rat Swissprot Link
http://www.uniprot.org/uniprot/P10980
免疫原
The antiserum was produced against synthesized peptide derived from human CHRM2. AA range:185-234
特异性
mAChR M2 Polyclonal Antibody detects endogenous levels of mAChR M2 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008],
细胞定位
Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Phosphorylation in response to agonist binding promotes receptor internalization. .
信号通路
Calcium;Neuroactive ligand-receptor interaction;Regulates Actin and Cytoskeleton;
功能
disease:Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.,function:The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.,polymorphism:Genetic variations in CHRM2 can influence susceptibility to alcoholism [MIM:103780].,similarity:Belongs to the G-protein coupled receptor 1 family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
