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Cleaved-Factor VII LC (R212) Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PC0081
产品名称
Cleaved-Factor VII LC (R212) Rabbit Polyclonal Antibody
别名
F7; Coagulation factor VII; Proconvertin; Serum prothrombin conversion accelerator; SPCA; Eptacog alfa
类别
常规抗体
基因名称
F7
蛋白名称
Coagulation factor VII
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
2155
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2155
Human Swissprot No.
P08709
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P08709/entry
Mouse Swissprot No.
P70375
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P70375
免疫原
The antiserum was produced against synthesized peptide derived from human FA7. AA range:171-220
特异性
Cleaved-Factor VII LC (R212) Polyclonal Antibody detects endogenous levels of fragment of activated Factor VII LC protein resulting from cleavage adjacent to R212.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
预测分子量
17kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015],
组织表达
Plasma.
细胞定位
Secreted.
信号通路
Complement and coagulation cascades;
功能
catalytic activity:Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.,disease:Defects in F7 are the cause of factor VII deficiency [MIM:227500]. Factor VII deficiency is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.,function:Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.,online information:Factor VII entry,online information:The Singapore human mutation and polymorphism database,pharmaceutical:Available under the names Niastase or Novoseven (Novo Nordisk). Used for the treatment of bleeding episodes in hemophilia A or B patients with antibodies to coagulation factors VIII or IX.,polymorphism:Individuals with the Q allele (Gln-413) seems to have a decreased susceptibility to myocardial infarction.,PTM:The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.,PTM:The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 Gla (gamma-carboxy-glutamate) domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 2 EGF-like domains.,subunit:Heterodimer of a light chain and a heavy chain linked by a disulfide bond.,tissue specificity:Plasma.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FA7 (light chain,Cleaved-Arg212) Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, treated with eto 25uM 24h, using FA7 (light chain,Cleaved-Arg212) Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Cleaved-Factor VII LC (R212) Polyclonal Antibody

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