产品名称
LIMK-2 Rabbit Polyclonal Antibody
别名
LIMK2; LIM domain kinase 2; LIMK-2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3985
Human Swissprot No.
P53671
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P53671/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16886
Mouse Swissprot No.
O54785
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54785
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29524
Rat Swissprot Link
http://www.uniprot.org/uniprot/P53670
免疫原
The antiserum was produced against synthesized peptide derived from human LIMK2. AA range:249-298
特异性
LIMK-2 Polyclonal Antibody detects endogenous levels of LIMK-2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Hepatoma,Lung,Ovary,
细胞定位
Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .; [Isoform LIMK2a]: Cytoplasm . Nucleus .; [Isoform LIMK2b]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus . Mainly present in the cytoplasm and is scarcely translocated to the nucleus. .
信号通路
Axon guidance;Fc gamma R-mediated phagocytosis;Regulates Actin and Cytoskeleton;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.,PTM:Phosphorylated on serine and/or threonine residues by ROCK1.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 LIM zinc-binding domains.,subcellular location:Isoform LIMK2a is distributed in the cytoplasm and the nucleus.,subcellular location:Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.,subunit:Binds ROCK1 and LKAP. Interacts with PARD3. Interacts with NISCH.,tissue specificity:Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
