产品名称
LARG Rabbit Polyclonal Antibody
别名
ARHGEF12; KIAA0382; LARG; Rho guanine nucleotide exchange factor 12; Leukemia-associated RhoGEF
蛋白名称
Rho guanine nucleotide exchange factor 12
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23365
Human Swissprot No.
Q9NZN5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9NZN5/entry
Mouse Swissprot No.
Q8R4H2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R4H2
免疫原
The antiserum was produced against synthesized peptide derived from human ARHGEF12. AA range:449-498
特异性
LARG Polyclonal Antibody detects endogenous levels of LARG protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],
组织表达
Ubiquitously expressed. Isoform 2 is found in jejunum and testis.
细胞定位
Cytoplasm . Membrane . Translocated to the membrane upon stimulation. .
信号通路
Regulation of Actin Dynamics; AMPK
功能
disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with MLL.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain with IGF1R beta subunit.,tissue specificity:Ubiquitously expressed. Isoform 2 is found in jejunum and testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
