产品名称
KLHL3 Rabbit Polyclonal Antibody
别名
KLHL3; KIAA1129; Kelch-like protein 3
蛋白名称
Kelch-like protein 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26249
Human Swissprot No.
Q9UH77
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9UH77/entry
Mouse Swissprot No.
E0CZ16
Mouse Swissprot Link
http://www.uniprot.org/uniprot/E0CZ16
免疫原
The antiserum was produced against synthesized peptide derived from human KLHL3. AA range:10-59
特异性
KLHL3 Polyclonal Antibody detects endogenous levels of KLHL3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012],
细胞定位
Cytoplasm, cytoskeleton . Cytoplasm, cytosol .
功能
function:Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subunit:Interacts with cul3.,tissue specificity:Seems to be expressed in a variety of tissues.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
