产品名称
KIF4A Rabbit Polyclonal Antibody
别名
KIF4A; KIF4; Chromosome-associated kinesin KIF4A; Chromokinesin-A
蛋白名称
Chromosome-associated kinesin KIF4A
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24137
Human Swissprot No.
O95239
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O95239/entry
免疫原
The antiserum was produced against synthesized peptide derived from human KIF4A. AA range:1171-1220
特异性
KIF4A Polyclonal Antibody detects endogenous levels of KIF4A protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
kinesin family member 4A(KIF4A) Homo sapiens This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010],
组织表达
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
细胞定位
Nucleus matrix . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Midbody . Chromosome . Associates with chromosomes at all stage of mitosis (PubMed:11736643, PubMed:15297875, PubMed:15625105). Chromatin localization is dependent on iron-sulfur cluster binding (PubMed:29848660). In anaphase, associates with the mitotic spindle midzone (PubMed:15297875). In telophase and cytokinesis, co-localizes with CIAO2B at the spindle midzone and midbody (PubMed:29848660, PubMed:15297875). Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase (PubMed:15297875, PubMed:15625105). Does not localize to the nucleolus (PubMed:11736643). .
功能
function:Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the kinesin-like protein family.,similarity:Belongs to the kinesin-like protein family. Chromokinesin subfamily.,similarity:Contains 1 kinesin-motor domain.,subcellular location:Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.,subunit:Interacts with unphosphorylated PRC1 during late mitosis.,tissue specificity:Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.,tissue specificity:Specifically expressed in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
