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KIF20A Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT2470
产品名称
KIF20A Rabbit Polyclonal Antibody
别名
KIF20A; MKLP2; RAB6KIFL; Kinesin-like protein KIF20A; GG10_2; Mitotic kinesin-like protein 2; MKlp2; Rab6-interacting kinesin-like protein; Rabkinesin-6
类别
常规抗体
基因名称
KIF20A
蛋白名称
Kinesin-like protein KIF20A
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
10112
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10112
Human Swissprot No.
O95235
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O95235/entry
Mouse Swissprot No.
P97329
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97329
免疫原
The antiserum was produced against synthesized peptide derived from human KIF20A. AA range:501-550
特异性
KIF20A Polyclonal Antibody detects endogenous levels of KIF20A protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
预测分子量
100kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
组织表达
Brain,Endothelial cell,Epithelium,Uterus,
细胞定位
Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Localizes to the spindle midzone during anaphase and telophase. .
功能
function:Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.,similarity:Belongs to the kinesin-like protein family.,similarity:Contains 1 kinesin-motor domain.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HUVEC cells, using KIF20A Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KIF20A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using KIF20A Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using KIF20A antibody.

Western Blot analysis of 293 cells using KIF20A Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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