BD-PT2440

JNK1/2/3 Rabbit Polyclonal Antibody

MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mi

常规抗体

MAPK8/9/10

Mitogen-activated protein kinase 8/9/10

WB

Human,Mouse,Rat,Chicken(testedbyourcustomer）,Fish,Pig

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

5599/5601/5602

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5599

P45983/P45984/P53779

http://www.uniprot.org/uniprotkb/P45983/entry

26419/26420

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26419

116554/50658/25272

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116554

P49185/P49186/P49187

http://www.uniprot.org/uniprot/P49185

The antiserum was produced against synthesized peptide derived from human SAPK/JNK. AA range:166-215

JNK1/2/3 Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

46+54kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl

Brain,Epithelium,Fetal brain,Lung,Pooled,Testis,

Cytoplasm . Nucleus . Cell junction, synapse . In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus. .

Toll_Like; Cell Growth; Stem cell pathway; Insulin Receptor; MAPK_ERK_Growth;MAPK_G_Protein; ErbB/HER; B Cell Receptor; SAPK_JNK; WNT;WNT-T CELL;β-Catenin

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.,function:JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.,function:Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).,online information:C-Jun N-terminal kinases entry,PTM:Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF (By similarity). Interacts with NFATC4.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.