BD-PT2422-IκB-ε Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IκB-ε Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT2422

产品名称

IκB-ε Rabbit Polyclonal Antibody

别名

NFKBIE; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon

类别

常规抗体

基因名称

NFKBIE

蛋白名称

NF-kappa-B inhibitor epsilon

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4794

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4794

Human Swissprot No.

O00221

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O00221/entry

Mouse Gene ID

18037

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18037

Mouse Swissprot No.

O54910

Mouse Swissprot Link

http://www.uniprot.org/uniprot/O54910

免疫原

The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon. AA range:131-180

特异性

IκB-ε Polyclonal Antibody detects endogenous levels of IκB-ε protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

45kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011],

组织表达

Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.

细胞定位

Cytoplasm .

信号通路

T_Cell_Receptor;B_Cell_Antigen;Neurotrophin;Adipocytokine;

功能

function:Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.,PTM:Serine phosphorylated; followed by proteasome-dependent degradation.,similarity:Belongs to the NF-kappa-B inhibitor family.,similarity:Contains 6 ANK repeats.,subunit:Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.,tissue specificity:Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IkappaB-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 5‘, using IkappaB-epsilon Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.