产品名称
IRAK-M Rabbit Polyclonal Antibody
别名
IRAK3; Interleukin-1 receptor-associated kinase 3; IRAK-3; IL-1 receptor-associated kinase M; IRAK-M
蛋白名称
Interleukin-1 receptor-associated kinase 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11213
Human Swissprot No.
Q9Y616
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y616/entry
Mouse Swissprot No.
Q8K4B2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K4B2
免疫原
The antiserum was produced against synthesized peptide derived from human IRAK3. AA range:491-540
特异性
IRAK-M Polyclonal Antibody detects endogenous levels of IRAK-M protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010],
组织表达
Expressed in eosinophils, dendritic cells and/or monocytes (at protein level) (PubMed:29686383). Expressed predominantly in peripheral blood lymphocytes (PubMed:10383454).
细胞定位
Cytoplasm . Nucleus . In dendritic cells, translocates into the nucleus upon IL33 stimulation. .
信号通路
Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Neurotrophin;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:Ser-293 is present instead of the conserved Asp which is expected to be an active site residue. Low level autophosphorylation activity has been reported in PubMed:10383454, while other authors describe this as an inactive kinase.,cofactor:Magnesium.,disease:Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.,function:Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed predominantly in peripheral blood lymphocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
