BD-PT2346-ILK Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ILK Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2346

产品名称

ILK Rabbit Polyclonal Antibody

别名

ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK

类别

常规抗体

基因名称

ILK

蛋白名称

Integrin-linked protein kinase

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3611

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3611

Human Swissprot No.

Q13418

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q13418/entry

Mouse Gene ID

16202

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16202

Mouse Swissprot No.

O55222

Mouse Swissprot Link

http://www.uniprot.org/uniprot/O55222

Rat Gene ID

170922

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=170922

Rat Swissprot No.

Q99J82

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q99J82

免疫原

The antiserum was produced against synthesized peptide derived from human ILK. AA range:212-261

特异性

ILK Polyclonal Antibody detects endogenous levels of ILK protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

预测分子量

42kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013],

组织表达

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

细胞定位

Cell junction, focal adhesion . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere .

信号通路

PPAR;Focal adhesion;Endometrial cancer;

功能

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.,PTM:Autophosphorylated on serine residues.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 ANK repeats.,subunit:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.,tissue specificity:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ILK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from rat liver cells, using ILK Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HUVECcells using ILK antibody.

Immunohistochemical analysis of paraffin-embedded Human ovary. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.