BD-PT2297-IK Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IK Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2297

产品名称

IK Rabbit Polyclonal Antibody

别名

IK; RED; RER; Protein Red; Cytokine IK; IK factor; Protein RER

类别

常规抗体

基因名称

蛋白名称

Protein Red

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3550

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3550

Human Swissprot No.

Q13123

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q13123/entry

Mouse Gene ID

24010

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24010

Mouse Swissprot No.

Q9Z1M8

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9Z1M8

Rat Gene ID

291659

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=291659

Rat Swissprot No.

Q66HG8

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q66HG8

免疫原

The antiserum was produced against synthesized peptide derived from human RED. AA range:508-557

特异性

IK Polyclonal Antibody detects endogenous levels of IK protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

66kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq, Jul 2008],

组织表达

Ubiquitous.

细胞定位

Nucleus . Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, spindle pole . Predominantly present throughout the nucleoplasm during prometaphase, metaphase and anaphase. Is also detected in nuclear foci that are not identical with Cajal bodies. Starts to accumulate at chromosomes during telophase, and is nearly exclusively associated with chromosomes in newly divided cells (PubMed:24252166). Colocalizes with MAD1L1 at mitotic spindle poles during metaphase and anaphase (PubMed:22351768). .

功能

caution:Was originally (PubMed:7970704) thought to be the IK factor, a cytokine involved in the negative regulatory pathway of constitutive MHC class II antigens expression.,developmental stage:Expressed at similar levels in fetal and adult tissues.,function:Not known. May bind to chromatin.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RED family.,tissue specificity:Ubiquitous.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HepG2, MCF-7, COLO, and HeLa cells, using RED Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from 293 cells using RED antibody.

Western Blot analysis of various cells using IK Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using IK antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.