BD-PT2272-Id4 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Id4 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2272

产品名称

Id4 Rabbit Polyclonal Antibody

别名

ID4; BHLHB27; DNA-binding protein inhibitor ID-4; Class B basic helix-loop-helix protein 27; bHLHb27; Inhibitor of DNA binding 4

类别

常规抗体

基因名称

ID4

蛋白名称

DNA-binding protein inhibitor ID-4

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3400

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3400

Human Swissprot No.

P47928

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P47928/entry

Mouse Gene ID

15904

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15904

Mouse Swissprot No.

P41139

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P41139

免疫原

The antiserum was produced against synthesized peptide derived from human ID4. AA range:1-50

特异性

Id4 Polyclonal Antibody detects endogenous levels of Id4 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

24kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011],

组织表达

Abdominal adipose tissue,Uterus,

细胞定位

Nucleus.

信号通路

TGF-beta;

功能

function:ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Heterodimer with other HLH proteins.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HepG2 cells, using ID4 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using ID4 antibody.

Western Blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.