BD-PT2267-IARS2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IARS2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2267

产品名称

IARS2 Rabbit Polyclonal Antibody

别名

IARS2; Isoleucine--tRNA ligase; mitochondrial; Isoleucyl-tRNA synthetase; IleRS

类别

常规抗体

基因名称

IARS2

蛋白名称

Isoleucine--tRNA ligase mitochondrial

推荐应用

反应种属

Human,Mouse,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

55699

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55699

Human Swissprot No.

Q9NSE4

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9NSE4/entry

Mouse Gene ID

381314

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=381314

Mouse Swissprot No.

Q8BIJ6

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8BIJ6

免疫原

The antiserum was produced against synthesized peptide derived from human IARS2. AA range:71-120

特异性

IARS2 Polyclonal Antibody detects endogenous levels of IARS2 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

114kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014],

组织表达

Brain,Epithelium,Liver,Placenta,Skin,

细胞定位

Mitochondrion matrix .

信号通路

Valine; leucine and isoleucine biosynthesis;Aminoacyl-tRNA biosynthesis;

功能

catalytic activity:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HepG2 cells, using IARS2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IARS2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from RAW264.7 and COS7 cells, using IARS2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using IARS2 Polyclonal Antibody diluted at 1：2000

Western Blot analysis of NIH-3T3 cells using IARS2 Polyclonal Antibody diluted at 1：2000