产品名称
HSL Rabbit Polyclonal Antibody
别名
LIPE; Hormone-sensitive lipase; HSL
蛋白名称
Hormone-sensitive lipase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3991
Human Swissprot No.
Q05469
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q05469/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16890
Mouse Swissprot No.
P54310
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P54310
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25330
Rat Swissprot Link
http://www.uniprot.org/uniprot/P15304
免疫原
The antiserum was produced against synthesized peptide derived from human HSL. AA range:518-567
特异性
HSL Polyclonal Antibody detects endogenous levels of HSL protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008],
细胞定位
Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). .
信号通路
Insulin Receptor; AMPK
功能
catalytic activity:Diacylglycerol + H(2)O = monoacylglycerol + a carboxylate.,catalytic activity:Monoacylglycerol + H(2)O = glycerol + a carboxylate.,catalytic activity:Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.,enzyme regulation:Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.,function:In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.,pathway:Glycerolipid metabolism; triacylglycerol degradation.,similarity:Belongs to the 'GDXG' lipolytic enzyme family.,subcellular location:Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.,subunit:Interacts with PTRF in the adipocyte cytoplasm.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
