产品名称
Cleaved-KLK11 (I54) Rabbit Polyclonal Antibody
别名
KLK11; PRSS20; TLSP; Kallikrein-11; hK11; Hippostasin; Serine protease 20; Trypsin-like protease
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11012
Human Swissprot No.
Q9UBX7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9UBX7/entry
Mouse Swissprot No.
Q9QYN3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9QYN3
免疫原
The antiserum was produced against synthesized peptide derived from human Kallikrein-11. AA range:35-84
特异性
Cleaved-KLK11 (I54) Polyclonal Antibody detects endogenous levels of fragment of activated KLK11 protein resulting from cleavage adjacent to I54.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009],
组织表达
Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
细胞定位
[Isoform 1]: Secreted.; [Isoform 2]: Golgi apparatus.
功能
function:Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.,PTM:About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.,similarity:Belongs to the peptidase S1 family.,similarity:Belongs to the peptidase S1 family. Kallikrein subfamily.,similarity:Contains 1 peptidase S1 domain.,tissue specificity:Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain; isoform 2 in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
