BD-PT2227

HRI Rabbit Polyclonal Antibody

EIF2AK1; HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2

常规抗体

EIF2AK1

Eukaryotic translation initiation factor 2-alpha kinase 1

WB

Human,Mouse,Monkey

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

27102

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27102

Q9BQI3

http://www.uniprot.org/uniprotkb/Q9BQI3/entry

15467

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15467

Q9Z2R9

http://www.uniprot.org/uniprot/Q9Z2R9

The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620

HRI Polyclonal Antibody detects endogenous levels of HRI protein.

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

71kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],

Expressed predominantly in erythroid cells (PubMed:20071449). Expressed at much lower levels in hepatocytes (at protein level) (PubMed:20071449).

cytoplasm,

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Hemin inactivates EIF2AK1 by promoting the formation of a disulfide-linked homodimer. Binding of nitric oxide (NO) to the heme iron in the N-terminal heme-binding domain activates the kinase activity, while binding of carbon monoxide (CO) suppresses kinase activity.,function:Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51'. Protein synthesis is inhibited at the level of initiation.,miscellaneous:Can bind 2 molecules of heme per polypeptide chain.,PTM:Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 HRM (heme regulatory motif) repeats.,subunit:Synthesized in an inactive form that binds to the N-terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Homodimer; non-covalently bound in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin.,tissue specificity:Detected in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, muscle and stomach.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.