BD-PT2220-HoxD12 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HoxD12 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2220

产品名称

HoxD12 Rabbit Polyclonal Antibody

别名

HOXD12; HOX4H; Homeobox protein Hox-D12; Homeobox protein Hox-4H

类别

常规抗体

基因名称

HOXD12

蛋白名称

Homeobox protein Hox-D12

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3238

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3238

Human Swissprot No.

P35452

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P35452/entry

Mouse Gene ID

15432

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15432

Mouse Swissprot No.

P23812

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P23812

免疫原

The antiserum was produced against synthesized peptide derived from human HOXD12. AA range:191-240

特异性

HoxD12 Polyclonal Antibody detects endogenous levels of HoxD12 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

28kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. The exact role of this gene has not been determined. [provided by RefSeq, Jul 2008],

组织表达

PCR rescued clones,

细胞定位

Nucleus.

功能

function:Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.,similarity:Belongs to the Abd-B homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO205 and HucEc cells, treated with serum 20% 15‘, using HOXD12 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using HoxD12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.