BD-PT2193-hnRNP A2/B1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

hnRNP A2/B1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2193

产品名称

hnRNP A2/B1 Rabbit Polyclonal Antibody

别名

HNRNPA2B1; HNRPA2B1; Heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2/B1

类别

常规抗体

基因名称

HNRNPA2B1

蛋白名称

Heterogeneous nuclear ribonucleoproteins A2/B1

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3181

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3181

Human Swissprot No.

P22626

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P22626/entry

Mouse Gene ID

100045000

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100045191

Mouse Swissprot No.

O88569

Mouse Swissprot Link

http://www.uniprot.org/uniprot/O88569

免疫原

The antiserum was produced against synthesized peptide derived from human hnRNP A2/B1. AA range:1-50

特异性

hnRNP A2/B1 Polyclonal Antibody detects endogenous levels of hnRNP A2/B1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

36+38kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq, Jul 2008],

组织表达

Brain,Cajal-Retzius cell,Cervix carcinoma,Epitheliu

细胞定位

Nucleus . Nucleus, nucleoplasm . Cytoplasm . Cytoplasmic granule . Secreted, extracellular exosome . Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661). Component of ribonucleosomes (PubMed:17289661). Not found in the nucleolus (PubMed:17289661). Found in exosomes following sumoylation (PubMed:24356509). .; [Isoform A2]: Nucleus . Cytoplasm . Predominantly nucleoplasmic, however is also found in the cytoplasm of cells in some tissues (PubMed:17289661). .

功能

function:Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subcellular location:Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.,subunit:Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using hnRNP A2/B1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using hnRNP A2/B1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using hnRNP A2/B1 Polyclonal Antibody diluted at 1：1000

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.