BD-PT2157-Histone H2B Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Histone H2B Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2157

产品名称

Histone H2B Rabbit Polyclonal Antibody

别名

H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s

类别

常规抗体

基因名称

H2BFS

蛋白名称

Histone H2B type F-S

推荐应用

反应种属

Human,Mouse,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

54145

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54145

Human Swissprot No.

P57053

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P57053/entry

免疫原

The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59

特异性

Histone H2B Polyclonal Antibody detects endogenous levels of Histone H2B protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

14kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

H2BFS is a Pseudogene. Histone H2B type 1-H is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. Diseases associated with H2BFS include endometrial stromal sarcoma. Among its related pathways are Packaging Of Telomere Ends. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BH.

细胞定位

Nucleus. Chromosome.

信号通路

Protein_Acetylation

功能

DNA packaging,?chromatin organization,?chromatin assembly or disassembly,?nucleosome assembly,?defense response,?response to bacterium,?chromatin assembly,?cellular macromolecular complex subunit organization,?cellular macromolecular complex assembly,?nucleosome organization,?defense response to bacterium,?macromolecular complex subunit organization,?chromosome organization,?macromolecular complex assembly,?protein-DNA complex assembly,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H2B Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Histone H2B Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of COS7 cells using Histone H2B Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.