产品名称
Hexim1 Rabbit Polyclonal Antibody
别名
HEXIM1; CLP1; EDG1; HIS1; MAQ1; Protein HEXIM1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10614
Human Swissprot No.
O94992
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O94992/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=192231
Mouse Swissprot No.
Q8R409
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R409
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=498008
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q5M9G1
免疫原
The antiserum was produced against synthesized peptide derived from human HEXIM1. AA range:181-230
特异性
Hexim1 Polyclonal Antibody detects endogenous levels of Hexim1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
细胞定位
Nucleus . Cytoplasm . Binds alpha-importin and is mostly nuclear (PubMed:16362050).
功能
domain:The coiled-coil domain mediates oligomerization.,function:Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.,induction:Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.,miscellaneous:Inhibits Tat activity which is required for HIV-1 transcription.,similarity:Belongs to the HEXIM family.,subcellular location:Binds alpha-importin and is mostly nuclear.,subunit:Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.,tissue specificity:Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
