产品名称
MYOF Rabbit Polyclonal Antibody
基因名称
MYOF FER1L3 KIAA1207
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/26509
Human Swissprot No.
Q9NZM1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9NZM1/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/226101
Mouse Swissprot No.
Q69ZN7
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q69ZN7
免疫原
Synthesized peptide derived from human MYOF AA range: 868-918
特异性
This antibody detects endogenous levels of MYOF at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008],
组织表达
Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
细胞定位
Cell membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein. Cytoplasmic vesicle membrane; Single-pass type II membrane protein. Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures. .
功能
cofactor:Binds calcium ions. The ions are bound to the C2 1 domain.,domain:The C2 domain 1 associates with lipid membranes in a calcium-dependent manner.,function:Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.,similarity:Belongs to the ferlin family.,similarity:Contains 5 C2 domains.,subcellular location:Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.,subunit:Interacts with DNM2 and KDR (By similarity). Interacts with EHD2; the interaction is direct.,tissue specificity:Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
