产品名称
GSTT1/4 Rabbit Polyclonal Antibody
别名
GSTT1; Glutathione S-transferase theta-1; GST class-theta-1; Glutathione transferase T1-1; GSTT4; Glutathione S-transferase theta-4; GST class-theta-4
蛋白名称
Glutathione S-transferase theta-1/Glutathione S-transferase theta-4
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2952
Human Swissprot No.
P30711/A8MPT4
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P30711/entry
免疫原
The antiserum was produced against synthesized peptide derived from human GSTT1/4. AA range:10-59
特异性
GSTT1/4 Polyclonal Antibody detects endogenous levels of GSTT1/4 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015],
组织表达
Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.
信号通路
Glutathione metabolism;Metabolism of xenobiotics by cytochrome P450;Drug metabolism;
功能
catalytic activity:RX + glutathione = HX + R-S-glutathione.,function:Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.,online information:The Singapore human mutation and polymorphism database,polymorphism:The GSTT1 gene is absent from 38% of the population. The presence or absence of the GSTT1 gene is coincident with the conjugator (GSST1+) and non-conjugator (GSTT1-) phenotypes respectively. The GSTT1+ phenotype can catalyze the glutathione conjugation of dichloromethane.,similarity:Belongs to the GST superfamily. Theta family.,similarity:Contains 1 GST C-terminal domain.,similarity:Contains 1 GST N-terminal domain.,subunit:Homodimer.,tissue specificity:Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
