BD-PT2064-GRK 2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GRK 2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2064

产品名称

GRK 2 Rabbit Polyclonal Antibody

别名

ADRBK1; BARK; BARK1; GRK2; Beta-adrenergic receptor kinase 1; Beta-ARK-1; G-protein coupled receptor kinase 2

类别

常规抗体

基因名称

ADRBK1

蛋白名称

Beta-adrenergic receptor kinase 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

156

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=156

Human Swissprot No.

P25098

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P25098/entry

Mouse Swissprot No.

Q99MK8

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q99MK8

Rat Gene ID

25238

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25238

Rat Swissprot No.

P26817

Rat Swissprot Link

http://www.uniprot.org/uniprot/P26817

免疫原

The antiserum was produced against synthesized peptide derived from human ARBK1. AA range:601-650

特异性

GRK 2 Polyclonal Antibody detects endogenous levels of GRK 2 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

80kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],

组织表达

Expressed in peripheral blood leukocytes.

细胞定位

Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .

信号通路

Chemokine;Endocytosis;

功能

catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HUVEC cells, using ARBK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using GRK 2 Polyclonal Antibody

Western Blot analysis of HuvEc cells using GRK 2 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.