产品名称
GPRC6A Rabbit Polyclonal Antibody
别名
GPRC6A; G-protein coupled receptor family C group 6 member A; hGPRC6A; G-protein coupled receptor GPCR33; hGPCR33
蛋白名称
G-protein coupled receptor family C group 6 member A
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=222545
Human Swissprot No.
Q5T6X5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q5T6X5/entry
Mouse Swissprot No.
Q8K4Z6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K4Z6
免疫原
The antiserum was produced against synthesized peptide derived from human GPRC6A. AA range:471-520
特异性
GPRC6A Polyclonal Antibody detects endogenous levels of GPRC6A protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010],
组织表达
Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detected in thymus, prostate, small intestine, tongue and colon. Isoform 1 and isoform 2 are expressed in kidney at the same level. Isoform 2 is expressed at lower level than isoform 1 in the other tissues.
细胞定位
Cell membrane ; Multi-pass membrane protein .
功能
function:Receptor that is activated by both amino acids and extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. Senses changes in the extracellular concentration of calcium ions, suggesting that it may mediate extracellular calcium-sensing responses in osteoblasts. Osteocalin, stimulates its activity in presence of calcium. Has a lower affinity for calcium than CASR. Also acts as a receptor for amino acids, with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine. Its affinity for amino acids suggests that it may act as a regulatory component of the urea cycle.,PTM:N-glycosylated.,similarity:Belongs to the G-protein coupled receptor 3 family.,subunit:Homodimer; disulfide-linked.,tissue specificity:Widely expressed. Expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not expressed in thymus, prostate, small intestine, tongue and colon. In kidney, isoform 1 and isoform 2 are expressed at the same level, while in other tissues, isoform 2 is expressed at low level.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
