产品名称
Glucosidase IIα Rabbit Polyclonal Antibody
别名
GANAB; G2AN; KIAA0088; Neutral alpha-glucosidase AB; Alpha-glucosidase 2; Glucosidase II subunit alpha
蛋白名称
Neutral alpha-glucosidase AB
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23193
Human Swissprot No.
Q14697
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q14697/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14376
Mouse Swissprot No.
Q8BHN3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BHN3
免疫原
The antiserum was produced against synthesized peptide derived from human GANAB. AA range:242-291
特异性
Glucosidase IIα Polyclonal Antibody detects endogenous levels of Glucosidase IIα protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes the alpha subunit of glucosidase II and a member of the glycosyl hydrolase 31 family of proteins. The heterodimeric enzyme glucosidase II plays a role in protein folding and quality control by cleaving glucose residues from immature glycoproteins in the endoplasmic reticulum. Expression of the encoded protein is elevated in lung tumor tissue and in response to UV irradiation. Mutations in this gene cause autosomal-dominant polycystic kidney and liver disease. [provided by RefSeq, Jul 2016],
组织表达
Detected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).
细胞定位
Endoplasmic reticulum . Golgi apparatus . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
信号通路
N-Glycan biosynthesis;
功能
catalytic activity:Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.,function:Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.,pathway:Glycan metabolism; N-glycan metabolism.,similarity:Belongs to the glycosyl hydrolase 31 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC.,tissue specificity:Detected in placenta.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
