产品名称
GK1 Rabbit Polyclonal Antibody
别名
GK; Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2710
Human Swissprot No.
P32189
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P32189/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14933
Mouse Swissprot No.
Q64516
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q64516
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=79223
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63060
免疫原
The antiserum was produced against synthesized peptide derived from human GK. AA range:461-510
特异性
GK1 Polyclonal Antibody detects endogenous levels of GK1 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011],
组织表达
Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
细胞定位
Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
信号通路
Glycerolipid metabolism;PPAR;
功能
catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.,tissue specificity:Highly expressed in the liver, kidney and testis. Isoforms 2 and 3 are expressed specifically in testis and fetal liver, but not in the adult liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
