产品名称
GCSc-γ Rabbit Polyclonal Antibody
别名
GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
蛋白名称
Glutamate--cysteine ligase catalytic subunit
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2729
Human Swissprot No.
P48506
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P48506/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14629
Mouse Swissprot No.
P97494
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97494
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25283
Rat Swissprot Link
http://www.uniprot.org/uniprot/P19468
免疫原
The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315
特异性
GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],
组织表达
Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus,
细胞定位
cytosol,integral component of membrane,glutamate-cysteine ligase complex,
信号通路
Glutathione metabolism;
功能
catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
