产品名称
Caspase-14 Rabbit Polyclonal Antibody
别名
CASP14; Caspase-14; CASP-14
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23581
Human Swissprot No.
P31944
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P31944/entry
Mouse Swissprot No.
O89094
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O89094
免疫原
Synthesized peptide derived from Caspase-14 . at AA range: 110-190
特异性
Caspase-14 Polyclonal Antibody detects endogenous levels of Caspase-14 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008],
组织表达
Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846).
细胞定位
Cytoplasm . Nucleus .
功能
function:Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.,induction:In undifferentiated keratinocytes under postconfluency growth conditions (in vitro).,similarity:Belongs to the peptidase C14A family.,subunit:Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits.,tissue specificity:Expressed in kerotinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
