BD-PT1860-GATA-2/3 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GATA-2/3 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1860

产品名称

GATA-2/3 Rabbit Polyclonal Antibody

别名

GATA3; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; GATA2; Endothelial transcription factor GATA-2; GATA-binding protein 2

类别

常规抗体

基因名称

GATA2/GATA3

蛋白名称

rans-acting T-cell-specific transcription factor GATA-2/3

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

2625

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2625

Human Swissprot No.

P23771/P23769

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P23771/entry

Mouse Gene ID

14462/14461

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14462

免疫原

The antiserum was produced against synthesized peptide derived from human GATA3. AA range:274-323

特异性

GATA-2/3 Polyclonal Antibody detects endogenous levels of GATA-2/3 protein.

稀释度

WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

参考分子量

48kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009],

组织表达

T-cells and endothelial cells.

细胞定位

Nucleus.

功能

disease:Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.,function:Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.,similarity:Contains 2 GATA-type zinc fingers.,tissue specificity:T-cells and endothelial cells.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using GATA3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using GATA3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of mouse-lung lysis using GATA-2/3 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human breast. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.