产品名称
GAK Rabbit Polyclonal Antibody
别名
GAK; Cyclin-G-associated kinase
蛋白名称
Cyclin-G-associated kinase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2580
Human Swissprot No.
O14976
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O14976/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=231580
Mouse Swissprot No.
Q99KY4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99KY4
免疫原
The antiserum was produced against synthesized peptide derived from human GAK. AA range:101-150
特异性
GAK Polyclonal Antibody detects endogenous levels of GAK protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
cyclin G associated kinase(GAK) Homo sapiens In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015],
组织表达
Ubiquitous. Highest in testis.
细胞定位
Cytoplasm, perinuclear region . Golgi apparatus, trans-Golgi network . Cell junction, focal adhesion . Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 C2 tensin-type domain.,similarity:Contains 1 J domain.,similarity:Contains 1 phosphatase tensin-type domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.,tissue specificity:Ubiquitous. Highest in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
