产品名称
Frizzled-8 Rabbit Polyclonal Antibody
别名
FZD8; Frizzled-8; Fz-8; hFz8
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8325
Human Swissprot No.
Q9H461
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9H461/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14370
Mouse Swissprot No.
Q61091
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61091
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100909849
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q498S8
免疫原
The antiserum was produced against synthesized peptide derived from human FZD8. AA range:486-535
特异性
Frizzled-8 Polyclonal Antibody detects endogenous levels of Frizzled-8 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
frizzled class receptor 8(FZD8) Homo sapiens This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008],
组织表达
Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
细胞定位
Membrane; Multi-pass membrane protein. Golgi apparatus . Cell membrane ; Multi-pass membrane protein . Colocalizes with GOPC at the Golgi apparatus. .
信号通路
WNT;WNT-T CELLMelanogenesis;Pathways in cancer;Colorectal cancer;Basal cell carcinoma;
功能
domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,domain:The PDZ-binding motif mediates interaction with GOPC.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.,similarity:Belongs to the G-protein coupled receptor Fz/Smo family.,similarity:Contains 1 FZ (frizzled) domain.,subunit:Interacts with GOPC. Interacts with RSPO1 and RSPO3.,tissue specificity:Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
