产品名称
Frizzled-5/8 Rabbit Polyclonal Antibody
别名
FZD5; C2orf31; Frizzled-5; Fz-5; hFz5; FzE5; FZD8; Frizzled-8; Fz-8; hFz8
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8325
Human Swissprot No.
Q13467/Q9H461
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13467/entry
Mouse Gene ID
14367/14370
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14367
Rat Gene ID
317674/100909849
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=317674
Rat Swissprot No.
Q8CHL0/Q498S8
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q8CHL0
免疫原
The antiserum was produced against synthesized peptide derived from human FZD8. AA range:31-80
特异性
Frizzled-5/8 Polyclonal Antibody detects endogenous levels of Frizzled-5/8 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
frizzled class receptor 5(FZD5) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008],
组织表达
Oesophageal carcinoma,Retina,
细胞定位
Cell membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cell junction, synapse . Perikaryon . Cell projection, dendrite . Cell projection, axon . Localized at the plasma membrane and also found at the Golgi. .
信号通路
WNT;WNT-T CELLMelanogenesis;Pathways in cancer;Colorectal cancer;Basal cell carcinoma;
功能
domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,domain:The PDZ-binding motif mediates interaction with GOPC.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.,similarity:Belongs to the G-protein coupled receptor Fz/Smo family.,similarity:Contains 1 FZ (frizzled) domain.,subcellular location:Localized at the plasma membrane and also found at the Golgi.,subunit:Interacts with GOPC.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
