产品名称
Frizzled-2 Rabbit Polyclonal Antibody
别名
FZD2; Frizzled-2; Fz-2; hFz2; FzE2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2535
Human Swissprot No.
Q14332
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q14332/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=57265
Mouse Swissprot No.
Q9JIP6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JIP6
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64512
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q08464
免疫原
The antiserum was produced against synthesized peptide derived from human FZD2. AA range:201-250
特异性
Frizzled-2 Polyclonal Antibody detects endogenous levels of Frizzled-2 protein.
稀释度
WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
frizzled class receptor 2(FZD2) Homo sapiens This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010],
组织表达
Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.
细胞定位
Membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein .
信号通路
WNT;WNT-T CELLMelanogenesis;Pathways in cancer;Colorectal cancer;Basal cell carcinoma;
功能
domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.,similarity:Belongs to the G-protein coupled receptor Fz/Smo family.,similarity:Contains 1 FZ (frizzled) domain.,tissue specificity:Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
