BD-PC0014-Cleaved-Caspase-9 p35 (D315) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Cleaved-Caspase-9 p35 (D315) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PC0014

产品名称

Cleaved-Caspase-9 p35 (D315) Rabbit Polyclonal Antibody

别名

CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

类别

常规抗体

基因名称

CASP9

蛋白名称

Caspase9

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

842

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=842

Human Swissprot No.

P55211

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P55211/entry

免疫原

The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:266-315

特异性

Cleaved-Caspase-9 p35 (D315) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p35 protein resulting from cleavage adjacent to D315.

稀释度

WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300

预测分子量

35 46kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

组织表达

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

细胞定位

nucleus,mitochondrion,cytosol,apoptosome,

信号通路

p53;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;VEGF;Alzheimer's disease;Parkinson's disease;Amyotrophic lateral sclerosis (ALS);Huntington's disease;Pathways in cancer;Colorectal cancer;Pancreatic cancer;Endometrial cancer;Prostate ca

功能

catalytic activity:Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).,function:Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.,online information:Caspase-9 entry,PTM:Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.,tissue specificity:Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 （Cleaved-Asp315） Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60‘, using Caspase 9 （Cleaved-Asp315） Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast tissue. 1,Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody diluted at 1：1000

Western Blot analysis of 293 cells using Cleaved-Caspase-9 p35 （D315） Polyclonal Antibody diluted at 1：1000