BD-PT1720-Fli-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Fli-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1720

产品名称

Fli-1 Rabbit Polyclonal Antibody

别名

FLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB

类别

常规抗体

基因名称

FLI1

蛋白名称

Friend leukemia integration 1 transcription factor

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

2313

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2313

Human Swissprot No.

Q01543

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q01543/entry

Mouse Gene ID

14247

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14247

Mouse Swissprot No.

P26323

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P26323

免疫原

The antiserum was produced against synthesized peptide derived from human FLI1. AA range:291-340

特异性

Fli-1 Polyclonal Antibody detects endogenous levels of Fli-1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

预测分子量

50kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],

组织表达

Blood,Bone marrow,Lymph,Placenta,

细胞定位

Nucleus .

功能

disease:A chromosomal aberration involving FLI1 is a cause of Ewing sarcoma [MIM:133450]. Translocation t(11;22)(q24;q12) with EWS.,function:Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.,miscellaneous:Located on a fragment of chromosome 11 flanked on the centromeric side by the acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation breakpoint and on the telomeric side by the Ewing- and neuroepithelioma-associated t(11;22) (q24;q12) breakpoint.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,similarity:Contains 1 PNT (pointed) domain.,subunit:Can form homodimers or heterodimers with ETV6/TEL1.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FLI1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using FLI1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Fli-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of 3T3 cells using Fli-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.