产品名称
Cleaved-Caspase-6 p18 (D179) Rabbit Polyclonal Antibody
别名
CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=839
Human Swissprot No.
P55212
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P55212/entry
Mouse Swissprot No.
O08738
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O08738
Rat Swissprot Link
http://www.uniprot.org/uniprot/O35397
免疫原
The antiserum was produced against synthesized peptide derived from human Caspase 6. AA range:130-179
特异性
Cleaved-Caspase-6 p18 (D179) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-6 p18 protein resulting from cleavage adjacent to D179.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015],
组织表达
Lung,Lymphocyte,T-cell,
细胞定位
Cytoplasm . Nucleus .
信号通路
Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;
功能
catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.,enzyme regulation:Activation is suppressed by phosphorylation at Ser-257.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.,PTM:Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
