BD-PT1704-Fhit Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Fhit Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1704

产品名称

Fhit Rabbit Polyclonal Antibody

别名

FHIT; Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

类别

常规抗体

基因名称

FHIT

蛋白名称

Bis(5'-adenosyl)-triphosphatase

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

2272

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2272

Human Swissprot No.

P49789

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P49789/entry

Mouse Swissprot No.

O89106

Mouse Swissprot Link

http://www.uniprot.org/uniprot/O89106

免疫原

The antiserum was produced against synthesized peptide derived from human FHIT. AA range:81-130

特异性

Fhit Polyclonal Antibody detects endogenous levels of Fhit protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

16kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009],

组织表达

Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.

细胞定位

Cytoplasm . Mitochondrion . Nucleus .

信号通路

Purine metabolism;Small cell lung cancer;Non-small cell lung cancer;

功能

catalytic activity:P(1)-P(3)-bis(5'-adenosyl) triphosphate + H(2)O = ADP + AMP.,cofactor:Divalent cations. Magnesium, but manganese and to a lesser extent calcium or cobalt can be substituted; but not zinc, cadmium or nickel.,disease:A chromosomal aberration involving FHIT is observed in early onset bilateral and multifocal clear cell renal carcinoma [MIM:144700]. Translocation t(3;8) (3p14.2).,disease:Associated with digestive tract cancers. Numerous tumor types are found to have aberrant forms of FHIT protein due to deletions in a coding region of chromosome 3p14.2 including the fragile site locus FRA3B.,function:Cleaves A-5'-PPP-5'A to yield AMP and ADP. Possible tumor suppressor for specific tissues.,mass spectrometry: PubMed:15007172,similarity:Contains 1 HIT domain.,subunit:Homodimer.,tissue specificity:Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using FHIT Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using FHIT Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Fhit Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.