BD-PT1689-FEN-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FEN-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT1689

产品名称

FEN-1 Rabbit Polyclonal Antibody

别名

FEN1; RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1

类别

常规抗体

基因名称

FEN1

蛋白名称

Flap endonuclease 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

2237

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2237

Human Swissprot No.

P39748

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P39748/entry

Mouse Swissprot No.

P39749

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P39749

Rat Gene ID

84490

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84490

Rat Swissprot No.

Q5XIP6

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q5XIP6

免疫原

The antiserum was produced against synthesized peptide derived from human FEN1. AA range:86-135

特异性

FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

42kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions

组织表达

Breast,Leukemic T-cell,Lung,

细胞定位

[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion .

信号通路

DNA replication;Base excision repair;Non-homologous end-joining;

功能

cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.,function:Endonuclease that cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Also possesses 5' to 3' exonuclease activity on niked or gapped double-stranded DNA, and exhibits RNase H activity.,PTM:Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.,similarity:Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.,subunit:Interacts with PCNA. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using FEN1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using FEN-1 Polyclonal Antibody diluted at 1：500

Western Blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.